Study and detection of ERG11 gene mutation in resistant - drug Candida albicans and relation with Iranian women infertility

Aim and Background: Candida  Albicans is the most important cause of  vulvovaginal candidiasis and resistance to azoles can occur via various mechanisms including, change in the ERG11 gene. This study aim was to identification  of  ERG11 gene mutations in drug  resistant Candida albicans isolated from patients with Candida vaginitis and its association with infertility in Iranian women.   Material and methods: From 100  patients with vaginitis Candida  Albicans species were isolated by using chromogen agar culture . The drug sensitivity was determined with standard protocol, diffusion disc test. Then extraction genome from resistance species and  was used PCR and sequencing for investigation of ERG11 gene mutations. For detection of sperm parameters disorders and relation with infertility, normal sperm was placed adjacent to sensitive and resistance Candida  Albicans by different concentrations and times.   Results: From 100  patients, 40 samples of Candida albicans were isolated that 2 samples were fluconazole and ketoconazole-resistant.  ERG11 gene mutations e in 2 samples were, conversion and insertion. In 10000 and 100000 CFU/ml concentrations, 4 hours and with 3 repeated, was observed significant relation to agglutination  and reduction in motility of the sperms (P<0.05).   Conclusion:  This study results showed that antibiotic resistance can be increased in Candida albicans with  mutation in the ERG11 gene and this Candida vaginitis infections, can associated with infertility in women.